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pETM6-Vv4CL-PhCHS-MsCHI

Plasmid

#73400

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-CmCHI

Plasmid

#73403

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-CmCHI

Plasmid

#73399

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tadh1

Plasmid

#127723

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pact1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Ppre3-ITE-Teno1

Plasmid

#127724

Purpose

Yeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppre3

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tdpp1

Plasmid

#127725

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pact1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Phta1-ITE-Tvma2

Plasmid

#127726

Purpose

Yeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Phta1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pfba1p-ACO-Tgpm1

Plasmid

#127731

Purpose

Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pykt6-ACO-Tprm9

Plasmid

#127732

Purpose

Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pykt6

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ptef1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Pzuo1-CSC-Tvma16

Plasmid

#127740

Purpose

Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pzuo1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppfy1

Available since

Availability

Academic Institutions and Nonprofits only

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eeBxb1

Plasmid

#222339

Purpose

Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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wtBxb1

Plasmid

#222337

Purpose

Plasmid for expressing wild type Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wild type Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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evoBxb1_IVT

Plasmid

#222341

Purpose

Plasmid for evolved Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Use

Template for in vitro transcription

Tags

Expression

Mutation

Promoter

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pREDCas9

Plasmid

#71541

Purpose

Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Inserts

Cas9
lambda red genes
plasmid curing system

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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