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pX601-mCherry

Plasmid

#84039

Purpose

Staphylococcus aureus (SaCas9) conjugated with mCherry

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TRE-MA4-GFP

Plasmid

#114380

Purpose

Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

Depositor

Yuet Wai Kan

Article

Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

MLL-AF4 fusion gene (AFF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Tet-on Advanced -BGH-PA

Plasmid

#114379

Purpose

Express reverse tetracycline inducible rtTA transcriptional activator

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Insert

rtTA and BGH-pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-PuroDTK.Neo

Plasmid

#72307

Purpose

PiggyBac Cassette contains puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Insert

Puromycin-DTK-neo

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG promoter

Available since

Feb. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG promoter

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET251-pUC 12xMS2V6 Loxp KANr Loxp

Plasmid

#104392

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-p2A-CreERT2-FRT-kan-FRT

Plasmid

#108454

Purpose

This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

mCherry-p2a-CreERT2

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ET_rhi145

Plasmid

#180427

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ET_rhi145

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-dsRed

Plasmid

#74152

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-dsRed

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-CFP

Plasmid

#74151

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-CFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

Plasmid

#180429

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ETh_rhi483

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-sfGFP

Plasmid

#74153

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFP

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-sfGFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

Plasmid

#44095

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

3xFlag, 6xGly linker, ADH1 transcription terminat…

Expression

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

Plasmid

#44094

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Expression

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER

Plasmid

#52687

Purpose

Same as 41025, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-GFP+LINKER

Use

Promoter replacement

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

Plasmid

#44089

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

3xFlag, 6xGly linker, ADH1 transcription terminat…

Expression

Mutation

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDZ207 (loxP KAN loxP 24xPP7SL MDN1)

Plasmid

#35191

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

loxP KAN loxP 24xPP7SL MDN1

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-YFP

Plasmid

#74154

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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