Addgene

We narrowed to 7,539 results for: Dos

Showing: 761 - 780 of 7539 results

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pSR78

Plasmid

#90210

Purpose

Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA

Depositor

Jeffrey Tabor

Article

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

CcaS
Cph8
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-SwiChRca-TS-EYFP

Plasmid

#55630

Purpose

Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

CaMKIIa

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Promoter

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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466 pME-Cherry

Plasmid

#26028

Depositor

Nathan Lawson

Article

Quillien et al Development. 2014 Apr;141(7):1544-52. do

Insert

mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-mRFP1-kanMX6

Plasmid

#133551

Purpose

Yeast fluorescent (RFP) vector with PTEF promoter.

Depositor

Erin O'Shea, Won-Ki Huh

Article

Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mLanYFP-polyA

Plasmid

#174021

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mLanYFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNa

Plasmid

#107980

Purpose

expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

full-length osteopontin (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-iRFP670-CaaX

Plasmid

#170464

Purpose

Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

iRFP670-CaaX

Use

Lentiviral

Tags

CaaX

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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MetNI (Lm)

Plasmid

#78316

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

Inserts

MetI
MetN

Use

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0547-luc2

Plasmid

#214665

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0547

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-myc-USP10

Plasmid

#184034

Purpose

Expresses myc-USP10 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

Insert

USP10 (USP10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMVf

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Contains D10A and H840A mutations

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc

Plasmid

#107982

Purpose

expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant c (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

splice variant

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-tdTomato-polyA

Plasmid

#174022

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

tdTomato

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJA291

Plasmid

#74487

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

puroR-mCherry-T2A-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAd5-B6 ∆E3-GFP

Plasmid

#179201

Purpose

Block 6 with deletion in E3 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6, with deletion in E3 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-3xFLAG-G

Plasmid

#127914

Purpose

a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen

Use

Tags

mNeonGreen-3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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