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Plasmid#65461Purposecontaining 1.3 units of the X-null HBV genome (subtype ayw)DepositorInsert1.3 units of the X-null HBV genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1932
Plasmid#142292PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertJUN (JUN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tandem p40p35
Plasmid#108665PurposeExpression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linkerDepositorUseTagsExpressionMammalianMutationIL-12 dimers have a Gly-Ser linkerPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-CeNL_pcDNA3
Plasmid#89513PurposeCyan color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertCeNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGF1-4eCOL2A1
Plasmid#97210PurposeLentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoterDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3mut
Plasmid#208704PurposeExpresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_gDA3mut in neuronsDepositorInsertGPCR activation based dopamine (DA) control sensor GRAB_gDA3mut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKikGR-C1
Plasmid#54656PurposeLocalization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593DepositorTypeEmpty backboneUseTagsmKikGRExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
miReporter-PGK
Plasmid#82477PurposemicroRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.DepositorInsertH2B-mCherry and H2B-Citrine. HygroR
UseTagsExpressionMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-LCMV-Arm53b
Plasmid#15796DepositorInsertLCMVArm53b envelope glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM3-IRES_BFP
Plasmid#179960PurposeExpression vector for human IFITM3. Co-expresses BFP controlled by an IRES element.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab7L1-c-Myc
Plasmid#79803PurposeExpress TagBFP labeled human Rab7L1.DepositorInsertRab7L1 (RAB29 Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ230-RR
Plasmid#108860PurposeLbCpf1 Gateway entry plasmid with G548R and K611R mutationsDepositorInsertLbCpf1-RR (LbCpf1 with G548R and K611R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R and K611R, Cpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI SF3B1 donor vector
Plasmid#65622PurposeDonor vector to generate Degron-KI DD-SF3B1DepositorInsertSF3B1 Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mEos2
Plasmid#105128PurposeExpresses human FGAMS-mEos2 fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_GCaMP6f
Plasmid#213943PurposeAAV construct expressing GCaMP6f driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_GCaMP6fDepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-BH
Plasmid#60515PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL6-H2B-iRFP720-puro
Plasmid#189992PurposeLentivirial plasmid for nuclear iRFP-720 expression with puromycin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro
Plasmid#141370PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF2972
Plasmid#144448PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR1H4 (NR1H4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEThT-CAHS3
Plasmid#90028PurposeTo express His-tagged CAHS3 in bacteriaDepositorInsertCAHS3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis WT
Plasmid#213603PurposeExpresses PLA2G15-6xHis in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PyronicSF-mRuby2/pBI-CMV1
Plasmid#124830PurposeHighly resposive GFP-based pyruvate nanosensor.DepositorInsertsPyronicSF
mRuby2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H4
Plasmid#42633DepositorInsertHuman H4 (H4C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro
Plasmid#141373PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagRFP-T-Lifeact-7
Plasmid#54586PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorTypeEmpty backboneUseTagsLifeAct -mTagRFP-TExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEThT-CAHS1
Plasmid#90027PurposeTo express His-tagged CAHS1 in bacteriaDepositorInsertCAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only