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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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KAT14-pDEST10

Plasmid

#118382

Purpose

HIS-tagged insect cell expression vector with KAT14.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Tags

HIS

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-2

Plasmid

#210130

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-1

Plasmid

#210013

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1, transient)

Plasmid

#197588

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

Plasmid

#208969

Purpose

Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

EcKlenow-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pIDK-HDAC1fl

Plasmid

#109158

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

p10

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-AIM2

Plasmid

#73958

Purpose

Express c-Myc-tagged human AIM2 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

Insert

absent in melanoma 2 (AIM2) (AIM2 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV iE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV14_NHP2L1(15.5kD)

Plasmid

#73065

Purpose

expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NHP2L1 (15.5kD) (SNU13 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-INK4A-zsGreen

Plasmid

#110728

Purpose

Mammalian Expression of p16INK4A IRES zsGreen

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES zsGreen (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR223 RpH-LAMP1-3xFLAG

Plasmid

#163019

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

Plasmid

#175261

Purpose

labelling of microtubule plus end and F-actin structures

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)

Use

Tags

mCherry (on iLifeact) and mNeonGreen (on EB3)

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1

Plasmid

#35170

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pIRES2-mCherry-p53 deltaN

Plasmid

#49243

Purpose

expresses human p53 deltaN and mCherry in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN (TP53 Human)

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

deltaN ( lacks 39 residues at the N-terminus)

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin

Plasmid

#118380

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

NLS

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-IKBalpha-wt

Plasmid

#15290

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pC1-IMS-oROS-HT

Plasmid

#216419

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-oROS-HT-CaaX

Plasmid

#216417

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT-CaaX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-dmito-oROS-HT

Plasmid

#216418

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

dmito-oROS-HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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