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pFA6a-His3MX6

Plasmid

#41596

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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AiP1070-Ai211 (TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2)

Plasmid

#181764

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

Kv2.1-tagged ChrimsonR-tdTomato and tTA2

Expression

Mammalian

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-TRP (V5 and HA)

Plasmid

#222488

Purpose

Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-mNeonGreen-WPRE

Plasmid

#223669

Purpose

Cre-dependently expresses mNeonGreen in astorocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mNeonGreen

Use

AAV and Cre/Lox

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

Plasmid

#127872

Purpose

Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-BmAgo3

Plasmid

#50559

Purpose

Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

BmAgo3

Tags

6xHIS and Flag

Expression

Insect

Promoter

OpIE2

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_AAV

Plasmid

#119924

Purpose

All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

AAV

Tags

NLS-3xHA-NLS and NLS-NLS

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMF1706

Plasmid

#125582

Purpose

Expresses peroxisomal roGFP2 (po-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

po-roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8m

Plasmid

#162372

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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