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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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p3E_he1a:eYFP

Plasmid

#113879

Purpose

p3E entry vector for Three Multisite Gateway

Depositor

Marnie Halpern

Insert

he1a (he1.1 Zebrafish)

Use

Zebrafish danio expression

Tags

eYFP

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pHCMV_EEE_E1E2

Plasmid

#207272

Purpose

Mammalian expression of Eastern equine encephalitis virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

EEE_E1E2 (EEEVgp3 Eastern equine encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIVT-eeBxb1

Plasmid

#232748

Purpose

IVT template for making modRNA encoding eeBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

V74A, E229K, V375I

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-c-fos-CytoTape-V5

Plasmid

#239429

Purpose

CytoTape signal monomer for recording c-fos promoter transcriptional activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

c-fos promoter

Available Since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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V2R-GFP

Plasmid

#208910

Purpose

encodes the human vasopressin receptor 2, tagged with GFP

Depositor

Christian Gruber

Article

Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

Insert

AVPR2 (AVPR2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

HaloTag

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

Plasmid

#169325

Purpose

Expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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