166,548 results

1. pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

   Plasmid

   #244072

   Purpose

   Cytosolic expression of green glucose sensor with non-responsive mIRFP

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

   Insert

   iGlucoSnFR2.mIRFP670nano3
   

   Use

   AAV

   Tags

   mIRFP670nano3

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0278

   Plasmid

   #142584

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARID2 (ARID2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

   Plasmid

   #218509

   Purpose

   integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mKate2
   

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Deletion of Plac_mrfp cassette, introduction of a…

   Promoter

   P23

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. iCAP-ROOT

   Plasmid

   #192262

   Purpose

   AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

   Depositor

   Li Ye

   Article

   Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

   Insert

   Synthetic construct for ROOT VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Plasmid

   #238244

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS_FtoG
   

   Expression

   Mammalian

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ExRai-AMPKAR

   Plasmid

   #192446

   Purpose

   Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 4xCSL-luciferase

   Plasmid

   #41726

   Depositor

   Raphael Kopan

   Article

   Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

   Insert

   4xCSL binding sites
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains a multimerized high affinity CSL binding…

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV MP-135 mRFP

   Plasmid

   #206927

   Purpose

   The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

   Depositor

   Markus Grompe

   Article

   Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

   Insert

   mRFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   MP-135

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221899

   Purpose

   Expresses alpha tubulin fused to mStayGold

   Depositor

   Janelia Integrative Imaging

   Insert

   mStayGold-aTubulin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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