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pSCAR_sgRNA_puro-mKate-lox2272

Plasmid

#162076

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Puro.Cre empty vector

Plasmid

#17408

Purpose

Lentiviral expression of Cre from the mouse PGK promoter

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

mouse PGK

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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dRED

Plasmid

#139836

Purpose

Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

RNase H1 protein contains D210N mutation. dCas9 …

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Raf-1 GST RBD 1-149

Plasmid

#13338

Depositor

Channing Der

Article

Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

Insert

Raf-1 (RAF1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-CreERT2

Plasmid

#14797

Purpose

Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pE-FLP

Plasmid

#45978

Purpose

Expresses yeast Flp driven by the constitutive promoter pE from phage P2

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Insert

flp

Use

Synthetic Biology

Expression

Bacterial

Mutation

flp driven by the constitutive promoter pE from p…

Promoter

pE (from phage P2)

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hnRNPU-V5

Plasmid

#35974

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)

Tags

V5

Expression

Mammalian

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET-hisT4DNALig

Plasmid

#216671

Purpose

Expresses his-tagged T4 DNA ligase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 DNA Ligase (30 T4 phage)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-flag S127A YAP

Plasmid

#27370

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Tags

flag

Expression

Mammalian

Mutation

S127A ( major phosphorylation site in YAP2)

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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