165,666 results

1. pH2B-miRFP680-N1

   Plasmid

   #197229

   Purpose

   Expresses the fusion protein of H2B-miRFP680 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   H2B-miRFP680
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HPSE-bio-His

   Plasmid

   #53407

   Purpose

   Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   HPSE (HPSE Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK-DTAG-CTERM-GFP

   Plasmid

   #185770

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-Rac1

   Plasmid

   #83950

   Purpose

   Rac1 FLIM donor for dynamic imaging of GTPase activity

   Depositor

   Ryohei Yasuda

   Article

   Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

   Insert

   ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP Kindlin2

   Plasmid

   #105305

   Purpose

   To study effect of Kindlin 2 on Cell/ECM adhesion

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rogi1-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179864

   Purpose

   Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-VSVG

   Plasmid

   #64084

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV glycoprotein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP2048 - pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220727

   Purpose

   AiE2543m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hSTING (N154S)

    Plasmid

    #214160

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N154S) (STING1 Human)
    

    Expression

    Mammalian

    Mutation

    N154S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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