166,152 results

1. HA-HIF1alpha-pcDNA3

   Plasmid

   #18949

   Depositor

   William Kaelin

   Article

   Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tom20-V5-FKBP-AP_pLX304

   Plasmid

   #120914

   Purpose

   Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   Tom20-V5-FKBP-AP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH47732

   Plasmid

   #48000

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCXLE-hOCT3/4

   Plasmid

   #27076

   Purpose

   Non-integrating (episomal) expression of human OCT3/4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Expression

   Mammalian

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL_PylRS(WT)-tRNA

   Plasmid

   #223512

   Purpose

   PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   araBAD, rrnB, and glnS and proK

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEC3135 (pERASE)

   Plasmid

   #220306

   Purpose

   Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSV Sport RXR alpha

   Plasmid

   #8882

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

   Insert

   RXR alpha (RXRA Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV mGAD65(delE1) GFP WPRE SV40pA

   Plasmid

   #177316

   Purpose

   To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

   Depositor

   Hirokazu Hirai

   Article

   Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

   Insert

   mGAD65(delE1) promoter, GABAergic interneurons specific promoter
   

   Use

   AAV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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