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pcDNA5-EGFP-AID-BubR1
Plasmid#47330Purposefor plasmid integration using the Flp-In system. AID at N-terminalDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
UseTagsAID and GFPExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ALK-F1174L
Plasmid#116108PurposeLentiviral expression of ALK F1174LDepositorInsertALK (ALK Human)
UseLentiviralTagsExpressionMutationF1174LPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PA50
Plasmid#84906Purposeinduce expression of dipeptide repeat PA in yeastDepositorInsertPA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT22-GluCNZ-6His
Plasmid#213504PurposeDesigned for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.DepositorInsertGlucanase (glucan endo-1,3-beta-glucosidase)
UseTags6His tagExpressionBacterialMutation1-36 Signal Sequence DeletionPromoterT7Available sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5CA(Q79L)
Plasmid#35138DepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationConstitutively Active- Q79LPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
SMA-Cherry
Plasmid#110209PurposeMapping of mesenchymal progenitor cells.DepositorInsertmCherry
UseTagsNoneExpressionMammalianMutationPromoterSmooth muscle alpha-actin and first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-mCherry (527)
Plasmid#70719PurposeExpression vector for mCherry purificationDepositorInsertmCherry
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2815
Plasmid#144244PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOSB (FOSB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only
