Addgene

We narrowed to 24,223 results for: sta

Showing: 7721 - 7740 of 24223 results

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pCB-CBEv1

Plasmid

#221138

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

lacIq-Ptac

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TRIM25_G1

Plasmid

#127119

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA TRIM25 (TRIM25 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-NLS-PTEN

Plasmid

#206984

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

tet-on promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-tag

Plasmid

#55180

Purpose

FLAG-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

FLAG

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pPIDNB2

Plasmid

#169902

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

Expression

Mammalian

Mutation

Promoter

Dox inducible

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A0103

Plasmid

#91000

Purpose

Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS-SV40-EGFP

Plasmid

#46919

Purpose

Target EGFP gene that is stably integrated into HEK293 genome

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

GFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pmiRFP680-tubulin

Plasmid

#136575

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP680

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP680-tubulin

Use

Tags

miRFP680

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Expression

Mutation

Promoter

U6 and CBh

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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eSrtA(2A-9) pET29b

Plasmid

#75145

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

Depositor

David Liu

Article

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Use

Tags

6x His

Expression

Bacterial

Mutation

S102C A104H E105D K138P K152I N160K K162H T164N K…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf6

Plasmid

#163598

Purpose

Expresses GDF-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf6 (Gdf6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Zeo-CnB

Plasmid

#191965

Purpose

Expression of CnB (calcineurin subunit beta).

Depositor

Luca Scorrano

Article

Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

Insert

Calcineurin subunit beta (PPP3CB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra2-Golgi-7

Plasmid

#57722

Purpose

Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBMN-dGBP1-TagBFP

Plasmid

#80087

Purpose

MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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P362-FBP_12

Plasmid

#139703

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pUC118-FLIP-FlpE-Puro

Plasmid

#84539

Purpose

a mock vector for generating FLIP-FlpE targeting vectors

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

FLIP-FlpE - Puro

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMM134

Plasmid

#127215

Purpose

BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, IPT, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPGKpuro

Plasmid

#11349

Depositor

Rudolf Jaenisch

Article

Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCEP4-YU2gp160

Plasmid

#100927

Purpose

Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (YU2) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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SaCas9

Plasmid

#188494

Purpose

Expresses FLAG tagged SaCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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