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Plasmid#217376PurposeLipid droplet localization of 6xHp of Spastin fused to mAppleDepositorInsertmApple-6x Hp NC
UseLentiviralTagsmAppleExpressionMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
RC(14-3-3ε)-LgBit
Plasmid#208926PurposeRecognition domain for in vitro RSK-activity sensorDepositorInsert14-3-3ε (YWHAQ Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FAB(ER-mito)
Plasmid#214427PurposeDetection of ER-mito contact siteDepositorInsertFRB-V5
UseTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Wdr20-V5
Plasmid#217622PurposeMammalian expression of V5 tagged Drosophila Wdr20DepositorInsertWDR20
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-ER
Plasmid#214413PurposeER localization of CFAST fused to Halotag-ERDepositorInsertrat cb5 ERM
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-mito
Plasmid#214415PurposeMitochondria localization of low-affinity NFAST fused to mApple-mitoDepositorInsertRspA-N
UseTagsmApple-mitoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-6xHp
Plasmid#214412PurposeLipid droplet localization of low-affinity NFAST fused to mApple-6xHpDepositorInsertRspA-N
UseTagsmApple-6xHpExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCBE-NNG-P2A-puro
Plasmid#214355PurposeExpresses SlugCBE-NNG and puromycin resistance genesDepositorInsertSlugCBE-NNG and puromycin resistance genes
UseTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9HF1-P2A-puro
Plasmid#214357PurposeExpresses SpCas9HF1 and puromycin resistance genesDepositorInsertSpCas9HF1 and puromycin resistance genes
UseTagsExpressionMammalianMutationN497A/R661A/Q695A/Q926APromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4(1-147)
Plasmid#195029PurposeThe control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vectorDepositorInsertGAL4 (1-147)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 Prg4 promoter-luciferase
Plasmid#195034PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-FR-2Strep
Plasmid#210312PurposeExpresses the C terminal of SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-EGFP
Plasmid#210318PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 N-2Strep
Plasmid#210376PurposeExpresses SARS-CoV-2 N protein fused with 2Strep in mammalian cellsDepositorInsertSARS-COV-2 N (N SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.5
Plasmid#208316PurposeExpresses ABE-RA5.5 in mammalian cellsDepositorInsertTadA-RA5.5-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PGUS
Plasmid#207827PurposeFLAG-tagged PGUS driven by CMV promoterDepositorInsertPGUS
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
REEP6-2Strep
Plasmid#210351PurposeExpresses REEP6 fused with 2Strep in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-398)-F394D/M396D/F397D
Plasmid#210848PurposeMammalian expression of ATG13 (363-398) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationF394D/M396D/F397DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-450)-F394D/F397D /E403K
Plasmid#210850PurposeMammalian expression of ATG13 (363-450) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationF394D/F397D /E403KPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only