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pEGFP-Nck1

Plasmid

#45903

Depositor

Louise Larose

Article

Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

Insert

Nck-1 (NCK1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-PRKRA

Plasmid

#31571

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Use

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBV-Luc mut MBS1-4

Plasmid

#16565

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS1-4

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pCCl-MCS_miniCMV_TagBFP

Plasmid

#134986

Purpose

The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

TagBFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMM4

Plasmid

#170359

Purpose

Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PCYC1_SEC61_mCherry_LEU2_TCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PCYC1

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Tags

Expression

Mutation

Rice codon optimized

Promoter

2x35S

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-nLuc

Plasmid

#199756

Purpose

Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβεζ

Plasmid

#187353

Purpose

Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPC-Halo

Plasmid

#175306

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPC (HNRNPC Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET14-GFP15_Encap

Plasmid

#86407

Purpose

Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 15aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pY2F

Plasmid

#53285

Purpose

Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pB-CAG-TEVp-IRES-mCherry

Plasmid

#174377

Purpose

For making TEVP cell line with hypBase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TEV Protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IRP1-Halo

Plasmid

#175305

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IRP1 (ACO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CaMKIIbeta(K43R)

Plasmid

#21225

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(K43R) (Camk2b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(K43R) Lab plasmid CF 0003.

Promoter

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_mcherry

Plasmid

#167862

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CaMKIIbeta(A303R)

Plasmid

#21224

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(A303R) (Camk2b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(A303R) (Lab plasmid CF 0002)

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only