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Plasmid#84351PurposeExpression of the human Huntingtin Exon1 protein containing 37Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PRKCI
Plasmid#45263DepositorInsertPKCI (PRKCI Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap2-SH2
Plasmid#46501DepositorInsertSlap2 (SLA2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 84-197PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA
Plasmid#128834PurposeKnock-in of tdTomato into mouse Utf1 locusDepositorInserttdTomato into Utf1 locus (Utf1 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(C)-SH2
Plasmid#46533DepositorInsertZap70(C) (ZAP70 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 154-267PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant
Plasmid#72616PurposeExpresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-Luci
Plasmid#31850DepositorInsertLuciferase RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS
Plasmid#162770PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX CblC-SH2
Plasmid#46414DepositorInsertCblc CBLC (CBLC Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 8-326PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs3-SH2
Plasmid#46506DepositorInsertSocs3 (SOCS3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 22-142PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-193a-3p
Plasmid#103305PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-193a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-193a-3p target (MIR193A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
S
Plasmid#167172PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cellsDepositorInsertsATP1A1-S
ATP1B1
UseTagsExpressionBacterial and InsectMutationPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Rescue Septin 11 mRNA
Plasmid#122022PurposeRescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.DepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationSilent mutations are in 5 consecutive codons (AUU…PromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-183-5p
Plasmid#103285PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-183-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-183-5p target (MIR183 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingTagsExpressionMutationPromotermNanogAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -0
Plasmid#173889PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-483-3p
Plasmid#103550PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-483-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-483-3p target (MIR483 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs1-SH2
Plasmid#46504DepositorInsertSocs1 (SOCS1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-166PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs4-SH2
Plasmid#46507DepositorInsertSocs4 (SOCS6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 376-498PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only