We narrowed to 8,680 results for: sgRNA

1. B-evoSpCas9-E526K

   Plasmid

   #207365

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-E526K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-evoSpCas9-V495M

   Plasmid

   #207363

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-V495M
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Y515N, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-HiFi SpCas9

   Plasmid

   #207362

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-HeFSpCas9-A1060R

   Plasmid

   #207372

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1060R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

   Promoter

   CBh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-HeFSpCas9-A1003K

   Plasmid

   #207371

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1003K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

   Promoter

   CBh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. B-HeFSpCas9-A848K

   Plasmid

   #207369

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A848K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B-HeFSpCas9-A661R

   Plasmid

   #207367

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. B-evoSpCas9-Q661R

   Plasmid

   #207366

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-Q661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. B-evoSpCas9-N515Y

   Plasmid

   #207364

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-N515Y
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9-EF-5Flnc

    Plasmid

    #174870

    Purpose

    CRISPR vector for generating Flnc STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Flnc (Flnc Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9-EF-5Usp5

    Plasmid

    #174873

    Purpose

    CRISPR vector for generating Usp5 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Usp5 (Usp5 Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9-EF-5Wnk1

    Plasmid

    #174874

    Purpose

    CRISPR vector for generating Wnk1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Wnk1 (Wnk1 Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX459_GRHL1-exon5

    Plasmid

    #177763

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX459_GRHL1-exon8

    Plasmid

    #177764

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

    Plasmid

    #176236

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176238

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX458_sgAgo2_Nterm

    Plasmid

    #170920

    Purpose

    Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo2 (Ago2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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