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Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianMutationPromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSIV-TRE-ASCL1
Plasmid#154883PurposeDoxycycline-dependently expresses human ASCL1.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPKR-FLAG-IG
Plasmid#101792PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PKRDepositorInsertPkr (Eif2ak2 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCBE4-gam
Plasmid#124449PurposeExpresses dead Cas9 (dCas9)-CBE4-gamDepositorInsertdCBE4-gam
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_tdtomato_reporter1_SP-2x
Plasmid#79370PurposeAssays activity of transcriptional activators fused to SP Cas9DepositorInserttdtomato
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHLP712
Plasmid#203741PurposeIntegration of OsTIR1(F74A)-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationCodon 74 changed from Phe to Ala (F74A)PromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag mHMGB2
Plasmid#31610DepositorInsertHMGB2 (Hmgb2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pp-mCAT1
Plasmid#83948PurposeThis plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectorsDepositorInsertmCAT1 (Slc7a1 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
1099 pcDNA GFP FKHR AAA
Plasmid#9023DepositorInsertFKHR AAA (FOXO1 Human)
UseTagsGFPExpressionMammalianMutationT24A S256A S319APromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-HA-human OCRL
Plasmid#22207DepositorInsertOculocerebrorenal syndrome of Lowe (OCRL Human)
UseRetroviralTagsHAExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP7 CS puroR
Plasmid#46752PurposeRetroviral vector that expresses catalytically inactive form of Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-NLS-SNAP
Plasmid#174879PurposePiggyBac transposon vector for expression of NLS-SNAPDepositorInsertNLS-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLGR002 LGR Cas9 guide vector
Plasmid#188320PurposeUniversal backbone for CRISPR/Cas9 sgRNA expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsPuroR-T2A-mTagBFP2ExpressionMammalianMutationPromoterpU6 5'CMV and EF1-alphaAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR-WT
Plasmid#159159PurposeExpresses DEPTOR in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only