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Plasmid#32072DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationDelete E746-A750, T790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro 3xFLAG Dna2 dm (nuc + hel)
Plasmid#31958DepositorInserthDna2 (DNA2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationChanged start ATG to AAA, and D294A and K671EPromoterAvailable sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro 3xFLAG Dna2 D294A (nuc)
Plasmid#31956DepositorInserthDna2 (DNA2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationChanged start ATG to AAA, and D294APromoterAvailable sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro 3xFLAG Dna2 K671E (hel)
Plasmid#31957DepositorInserthDna2 (DNA2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationChanged start ATG to AAA; K671EPromoterAvailable sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
c-Myc-5-HT2A
Plasmid#67944PurposeN-terminally c-myc tagged human 5-hydroxytryptamine receptor 2ADepositorInsertHTR2A (HTR2A Human)
UseTagsc-MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD2sE_SC14
Plasmid#219717PurposeDV2 stable E dimerDepositorInsertDV2 sE SC14
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationA259W, T262R, F279W, T280PPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD2sE_SC10
Plasmid#219716PurposeDV2 stable E dimer with fusion loop mutationDepositorInsertDV2 sE SC10
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, T280PPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ4M/TDP-43
Plasmid#104480Purposebacterial expression of TDP-43DepositorInsertTDP-43 (TARDBP Human)
UseTagsMBP-6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Flag-His-ATM wt
Plasmid#31985PurposeMammalian expression of human ATM with Flag and His tagsDepositorInsertATM wild-type (ATM Human)
UseTagsFlag and HISExpressionMammalianMutation3' UTR of 400 nucl.PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX330-mitf
Plasmid#69801PurposeUsed to make mitf knockout porcine cell lineDepositorInsertsmitf-sgRNA
SpCas9
UseTagsExpressionMammalianMutationPromoterChicken Beta-Actin and U6Available sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRN120
Plasmid#160696PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminatorDepositorInsertsCmYLCV::Luc+::AtHSP
Nos::WUS2::PinII
AtUbi10::STM::35S
UseLuciferase; Gemini viral replicon, plant developm…TagsExpressionMutationPromoterAtUbi10, CmYLCV, and NosAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone
Plasmid#216154PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5']DepositorInsertmScarlet with cryptic exon and C-terminal FLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(S387A)
Plasmid#220462PurposeExpress S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
UseTags3xFLAGExpressionMammalianMutationSerine 387 to alaninePromoterCMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CD-MPR-mCherry-RUSH
Plasmid#202798PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPRDepositorInsertM6PR (M6PR Human)
UseTagsmCherryExpressionMammalianMutationChanged 40 K (AAA) with R (AGA)PromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-C450A
Plasmid#22028PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hMC4R
Plasmid#179315PurposeExpress human melanocortin 4 receptor (GFP tagged) in mammalian cellsDepositorInsertmelanocortin 4 receptor (MC4R Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2K-LRL-IRES-Luc2
Plasmid#109230PurposeFor Tol2 transposon-mediated stable expression of LRL and IRES-luciferaseDepositorInsertLRL and Luc2
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only