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Plasmid#72266PurposeExpress gene of interest under EF-1 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits
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RIX-F2Oct4F3-GFP
Plasmid#216848PurposeThis plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diffDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterOct4Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDG459
Plasmid#100901PurposeSpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
UseTags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-INSR-V5/HIS
Plasmid#201980Purposeexpression of human INSR receptor tyrosine kinase in mammalian cellsDepositorInsertINSR receptor tyrosine kinase (INSR Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ4M/TDP-43
Plasmid#104480Purposebacterial expression of TDP-43DepositorInsertTDP-43 (TARDBP Human)
UseTagsMBP-6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-Mito4x-GCaMP6f
Plasmid#127870PurposeFluorescent reporter for mitochondrial matrix Ca2+DepositorInsert4 repeats of COX8 signal peptide plus GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only