Addgene

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Use

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Tags

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-jGCaMP8s-WPRE

Plasmid

#179256

Purpose

AAV-mediated expression of GCaMP8s under the CAG promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Use

Tags

MBP-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Plasmid

#226514

Purpose

Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

Depositor

Kai Johnsson

Article

Kühn et al bioRxiv 2024.08.28.610127

Insert

HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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