-
Plasmid#147604PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
UseTagsExpressionInsectMutationTwo non silent mutations (H628R and N659S) compar…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorInsertDmCup-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationY342A, L347A, I350L, P418S, K1045NPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y
Plasmid#148080PurposeInsect Expression of DmPAN3-dsRNAresDepositorInsertDmPAN3-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-510-Y10AL15A-V5His6_D
Plasmid#146152PurposeInsect Expression of Dm4E-T_1-510-Y10AL15ADepositorInsertDm4E-T_1-510-Y10AL15A (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW4
Plasmid#166710Purposeexpresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cellsDepositorInsertNEDD4L W576A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 576 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW1(193-226)
Plasmid#166701Purposeexpresses NEDD4L WW1 in bacterial cellsDepositorInsertNEDD4L WW1 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW2(385-418)
Plasmid#166702Purposeexpresses NEDD4L WW2 in bacterial cellsDepositorInsertNEDD4L WW2 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW3(497-530)
Plasmid#166703Purposeexpresses NEDD4L WW3 in bacterial cellsDepositorInsertNEDD4L WW3 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW4(548-581)
Plasmid#166704Purposeexpresses NEDD4L WW4 in bacterial cellsDepositorInsertNEDD4L WW4 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY153: Flag(SUZ12) WT HDR plasmid
Plasmid#170793PurposeWT homology directed repair plasmid for performing genome-editing on the SUZ12 LocusDepositorInsertSUZ12 (SUZ12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gLacZ.EFS-NS.H2B-RFP
Plasmid#170363PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH42
Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialMutationPromoternative promoter of sll0617Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd388-BPTF-sh28
Plasmid#83276PurposeshRNA targeting BPTFDepositorInsertshRNA to BPTF
UseAdenoviralTagsExpressionMutationPromoterpUC oriAvailable sinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-FLAG-SBP-STAG2 (Y1142fs)
Plasmid#73969PurposeSTAG2 cDNA (CCDS43990) Y1142fsDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationY1142fsPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (I885fs)
Plasmid#73966PurposeSTAG2 cDNA (CCDS43990) I885fsDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationI885fsPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only