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Plasmid#63183PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSW609 - N001
Plasmid#115993Purposeintermediate vector for GreenGate cloning method, contains modules from A-FDepositorInsertH-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseUnspecifiedTagsHDEL and SP(ER)ExpressionMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR
Plasmid#181832PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).DepositorInsertLyn-PlcR
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)
Plasmid#85038PurposeFor Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.DepositorInsertFRT-stop-FRT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase
Plasmid#163404PurposeExpresses BMPR-2 lacking the kinase domain in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LGC-53
Plasmid#86399PurposeMammalian expression of codon-optimized dopamine receptor LGC-53DepositorInsertLGC-53 (lgc-53 Synthetic, Nematode)
UseTagsExpressionMammalianMutationCodon optimized for mammalian expression; Signal …PromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-ATG5
Plasmid#99573PurposeExpresses Cas9 with sgRNA targeting Exon 7 of ATG5DepositorInsertgRNA targeting ATG5 (ATG5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa
Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa-SpCas9D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HBT-GCaMP6-HA
Plasmid#107433PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertGCaMP6S
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
Plasmid#89587PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.DepositorInsertnuclear localization signal (nls) turboRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro hTERT shRNA
Plasmid#10688DepositorInserthTERT shRNA (TERT Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG mEmerald-LAMP1
Plasmid#168513PurposeExpression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomesDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmEmeraldExpressionMammalianMutationPromoterCAGAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 CC
Plasmid#24403DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains the CC domain (aa 151-242)PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
TaRGET-ABE-C3.1 ver4.1
Plasmid#188251PurposeHypercompact adenine base editorsDepositorInsertTaRGET-ABE3.1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP
Plasmid#174876PurposePiggyBac transposon vector for expression of RNAPII Ser2ph mintbodyDepositorInsertRNAPII Ser2ph mintbody-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Beta-Globin-PTC39
Plasmid#130701PurposeExpresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)DepositorInsertBeta Globin (HBB Human)
UseTagsExpressionMammalianMutationPTC39PromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry
Plasmid#75470PurposeFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertReversed ChR2(H134R)-mCherry
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only