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pAC149-pCR8-dCas9VP160

Plasmid

#48221

Purpose

dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP160

Expression

Mutation

D10A;H840A nuclease-deficient

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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KIF1A(1-393)-GCN4-2xmCherry-EF(C)

Plasmid

#61665

Purpose

Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAdeltaIQ2

Plasmid

#35690

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Use

Tags

EGFP and GFPGenotRW

Expression

Mammalian

Mutation

The coding segment for aa 804-834 (corresponding …

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pEB3-miRFP703

Plasmid

#79994

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

EB3 (MAPRE3 Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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F-MED26

Plasmid

#15367

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-B2AR-APEX

Plasmid

#96848

Purpose

For expression of B2AR-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

B2AR-APEX (ADRB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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BCL9-BirA*

Plasmid

#109147

Purpose

To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

Depositor

Mariann Bienz

Article

van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

Insert

BCL9 (BCL9 Human)

Use

Tags

BirA(R118G)

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G972R

Plasmid

#174800

Purpose

Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G972R (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

G972R, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ143-ZmUbi

Plasmid

#138107

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBT3035

Plasmid

#122543

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_lmn-1::CemOrange2 (lmn-1 Synthetic, Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only
pBT3000

Plasmid

#122536

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

Plasmid

#187994

Purpose

Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

mucin2 D1D2D'D3 (MUC2 Human)

Use

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CCNB1-P2A-Hygro_Barcode

Plasmid

#170218

Purpose

Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CCNB1 (CCNB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Use

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-eLACCO2.1-NGR

Plasmid

#207989

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yuki Kambe

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU3m/LacZ

Plasmid

#66192

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

OsU3m (SpeI site destroyed)

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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