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pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Orf6

Plasmid

#157726

Purpose

mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Orf6 (ORF6 SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp15

Plasmid

#157720

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp14

Plasmid

#157717

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMTTH+mEGFP-H1.4ΔCTD

Plasmid

#157800

Purpose

Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4ΔCTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+LANA-H1.4CTD

Plasmid

#157798

Purpose

Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

LANA-H1.4CTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7 (zebrafish) C117S

Plasmid

#108915

Purpose

Express C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7

Use

Tags

Halo-TEV

Expression

Mammalian

Mutation

Cysteine 117 to Serine

Promoter

SP6

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Snrnp40

Plasmid

#134255

Purpose

Lentivector for Snrnp40 CRISPR knockout

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

Promoter

U6

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-Snrnp40

Plasmid

#134249

Purpose

Lentivector encoding Flag-tagged Snrnp40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416) (L313D I314K)

Plasmid

#121409

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to …

Promoter

CUP1 promoter

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSi-check2-hRac2 3'UTR

Plasmid

#97160

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

RAC 3'UTR (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-607

Plasmid

#92258

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-633

Plasmid

#92253

Purpose

Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,S166A/pET28b(+)

Plasmid

#83301

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Serine 166 …

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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