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Showing: 7621 - 7640 of 7946 results

  1. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  2. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid
    #180017
    Purpose
    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.
    Depositor
    Dirk Hockemeyer
    Article
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Insert
    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180017-plasmid-map-sequence-id-354058

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  10. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #156452
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    RAD21, AID (Rad21 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156452-plasmid-map-sequence-id-310171

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  18. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid
    #199263
    Purpose
    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199263-plasmid-map-sequence-id-389813

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Showing: 7621 - 7640 of 7946 results