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Showing: 7621 - 7640 of 10528 results

  2. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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  3. pEGFP_∆R13–DD-talin1 (1-2299)

    Plasmid
    #166125
    Purpose
    For expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.
    Depositor
    Vesa Hytönen
    Article
    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.
    Insert
    ∆R13–DD-Talin-1 (Tln1 Mouse)
    Use
    Cmv promoter
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    residues 1-2299, deletion of dimerization domain …
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166125-plasmid-map-sequence-id-330294

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  4. GFP-MIOX only

    Plasmid
    #166683
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    GFP-MIOX
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166683-plasmid-map-sequence-id-329938

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  5. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    UDH
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166679-plasmid-map-sequence-id-329863

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  6. wtVP1 only

    Plasmid
    #166673
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166673-plasmid-map-sequence-id-329825

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  7. p1A CbbL-

    Plasmid
    #162693
    Purpose
    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    CbbL K194M (inactive rubisco)
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162693-plasmid-map-sequence-id-324169

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Showing: 7621 - 7640 of 10528 results