Addgene

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYI047

Plasmid

#170843

Purpose

(pFRO6::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pFRO6::SMT2-FLAG

Use

Tags

Expression

Plant

Mutation

Promoter

FRO6p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI048

Plasmid

#170844

Purpose

(pGH3.17::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pGH3.17::SMT2-FLAG

Use

Tags

Expression

Plant

Mutation

Promoter

GH3.17p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI012

Plasmid

#170841

Purpose

pGH3.17::GUSPlus::tOCS

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GH3.17p

Use

Tags

Expression

Plant

Mutation

Promoter

GH3.17p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI046

Plasmid

#170842

Purpose

(pUBQ10::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pUBQ10::SMT2-FLAG::tOCS

Use

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_3EGFP

Plasmid

#185967

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-Egfp cassette in forward direction in Network Brick

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PR

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-tdTomato-WPRE

Plasmid

#187112

Purpose

FLP-dependent expression of tdTomato under EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJAK175

Plasmid

#178601

Purpose

pAP259 derived plasmid encoding a xylose inducible BitlucOpt

Depositor

Robert Fagan

Article

Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

Insert

Bitlucopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pxyl

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Ile6Met

Plasmid

#128687

Purpose

Expresses GB1 Ile6Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val29Met

Plasmid

#128688

Purpose

Expresses GB1 Val29Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Ala34Met

Plasmid

#128689

Purpose

Expresses GB1 Ala34Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val39Met

Plasmid

#128690

Purpose

Expresses GB1 Val39Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val54Met

Plasmid

#128691

Purpose

Expresses GB1 Val54Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Use

Tags

2XGFP

Expression

Worm

Mutation

Promoter

nlf-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2437

Plasmid

#179780

Purpose

Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1 (nlf-1 Nematode)

Use

Tags

no

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D

Plasmid

#146123

Purpose

Insect Expression of DmEDC3_1-440

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-440 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmEDC3_441-680_D

Plasmid

#146127

Purpose

Insect Expression of DmEDC3_441-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_441-680 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

3 mutations N162T, K221E and D418G compared to th…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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