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Plasmid#148840PurposeMammalian Expression of DmBamDepositorInsertDmBam (bam Fly)
UseTagsExpressionMammalianMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPhiC31-actIIORF4
Plasmid#190785PurposeThe PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoterDepositorInsertsT5 terminator
actII-4
kasOp*
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Lm-iPGM
Plasmid#180242PurposeBacterial expression plasmid for production of recombinant Leishmania mexicana iPGM His10DepositorInsertLeishmania mexicana iPGM-10His
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Mp-iPGM
Plasmid#180243PurposeBacterial expression plasmid for production of recombinant Mycoplasma pneumoniae iPGM His10DepositorInsertMycoplasma pneumoniae iPGM-10His
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Wb-iPGM
Plasmid#180246PurposeBacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10DepositorInsertWuchereria bancrofti iPGM-10His
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvM-DmBam_141-442_AG
Plasmid#148833PurposeBacterial Expression of DmBam_141-422DepositorInsertDmBam_141-422 (bam Fly)
UseTagsExpressionBacterialMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mPpp3cc-FLAG-6XHIS
Plasmid#179160PurposeExpression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
UseTags6XHIS and FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSS-GFP
Plasmid#163661PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSN-GFP
Plasmid#163663PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Gapdh
Plasmid#175938Purposeknockout mouse GapdhDepositorInsertGapdh gRNA (Gapdh Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC002
Plasmid#171139PurposeFor integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertsJ1-BBa_J23117-sfGFP
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterJ1-BBa_J23117 and Sp.pCas9, BBa_J23107Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPodA11
Plasmid#162843PurposeExpression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistanceDepositorInsertProtein Designed Pyocyanin demethylase
UseTagsTEV-cleavable 6x-His tagExpressionBacterialMutationA53N, I73T, A87V, M99V, A129T from WT PodAPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMC00007
Plasmid#166725Purposenon targeting sgRNADepositorInsertNon-targeting guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB
Plasmid#166410Purposeexpress BirA-hPOT1 ΔOB in mammalian cellsDepositorInsertPOT1 ΔOB (POT1 Human)
UseTagsMYC-flag-BirAExpressionMammalianMutationΔOBPromotercmvAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3G919C
Plasmid#120255PurposeExpression of ATP3-7 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
UseTagsExpressionYeastMutationG919 changed to C; Alanine 307 changed to ProlinePromoterATP3Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3mut
Plasmid#120256PurposeExpression of ATP3-67 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
UseTagsExpressionYeastMutationIsoleucine 304 changed to Asparagine; Alanine 307…PromoterATP3Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBP-GFP-Tac-TC
Plasmid#162506PurposeExpresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cellsDepositorInsertGFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
UseTagsGFP and SBPExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCdc20
Plasmid#160954PurposeCdc20 shRNA in pMKO.1 retroviral vectorDepositorInsertCdc20 shRNA (Cdc20 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only