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Plasmid#167205PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsmClover3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGL3-E-cadherin promoter
Plasmid#61798PurposeLuciferase reporter containing the mouse E-cadherin promoterDepositorInsert-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-G6
Plasmid#194589PurposeBacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertG6 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
Plasmid#217510PurposeKD Recombinase dependent conditional smGdP-10XV5 cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
UseTagssmGdP-10XV5ExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)
Plasmid#214881PurposeLentiviral vector encoding RfxCas13d targeting B2M guideDepositorInserthU6-crB2M-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
A2_uSFFV
Plasmid#153415PurposeExpresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoterDepositorInsertA*02:01-P2A-human beta2 microglobulin (B2M Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2 Nsp3TMC111A
Plasmid#204207PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx1ExpressionMammalianMutationCys to Ala in active site of PLproPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-HA-deLACCO1-NGR
Plasmid#208097PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gfaABC1D-HA-deLACCO1-NGR
Plasmid#208099PurposeControl biosensor for extracellular L-lactateDepositorInsertR-iLACCO1.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-GCaMP7f
Plasmid#135539PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7fDepositorInsertXph20
UseTagsGCaMP7fExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hAtg12
Plasmid#87869PurposeExpress a EGFP-human Atg12/Apg12 in mammalian cellsDepositorInsertATG12 (ATG12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-YY1
Plasmid#154943PurposeKnocking down of human YY1 through shRNA and amiRNA targeting YY1DepositorInsertshRNA and amiRNA against YY1 (YY1 Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA1-28-pA
Plasmid#55200PurposePlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV siteā¦ExpressionBacterialMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-T2TP
Plasmid#114725Purposeexpression in mammalian cellsDepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-VARNAM2-Kv2.1PR
Plasmid#195533PurposeCre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
TA1-DuET
Plasmid#213382PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertTA1 (TAAR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLdCN
Plasmid#84290PurposeExpress gRNA and Cas9 in Leishmania with Neomycin resistanceDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterL. donovani ribosome RNA promoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only