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Plasmid#107722PurposeFor expression of sgRNA from the U6 promoter with BFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLX208 CMV sTurboID (C)-HA-FRB-ERM
Plasmid#153007PurposeExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membraneDepositorInsertsTurboID (C) (G74)-ERM (Cb5)
UseLentiviralTagsFRB and HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Klotho (secreted)
Plasmid#17713DepositorInsertKlotho (KL Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV OMM-FKBP-V5-sTurboID (N)
Plasmid#153006PurposeExpresses split-TurboID N-terminal fragment targeted to outer mitochondrial membraneDepositorInsertOMM (Tom20)-sTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP2
Plasmid#42308DepositorInsertVAMP 2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat
Plasmid#169128PurposeAAV plasmid containing a mKate2 and a truncated PDE4D3DepositorInsertmKate2-PDE4DCatL
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationtruncated, Catalatic domain onlyPromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmbim6-V5
Plasmid#175150PurposeLentiviral expression of mouse Tmbim6-V5DepositorInsertTmbim6 (Tmbim6 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AktAR
Plasmid#181856PurposeGenetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AktAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorInsertCullin 4B (CUL4B Human)
UseTags3xmycExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pK170.AAV-TRE-Cre-WPRE (Supernova)
Plasmid#85040PurposeFor AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.DepositorInsertnlsCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 1-150
Plasmid#24389DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 1-150.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag hHMGB1
Plasmid#31609DepositorInsertHMGB1 (HMGB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS-RED-C1-Beclin1
Plasmid#24405DepositorInsertBeclin1 (BECN1 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
C1A-C1A-EYFP
Plasmid#61155PurposeDiacylglycerol (DAG) reporterDepositorInsertC1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
UseTagsEYFPExpressionMammalianMutationC1A from PKCG amino acids 26-89PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorInsertloxP-stop-loxP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 151-241
Plasmid#24390DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 151-241.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only