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amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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PB-SAM

Plasmid

#102559

Purpose

Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Inserts

MS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9 and N55K in MS2

Promoter

CAG and EF1A

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-ERKKTRClover

Plasmid

#59138

Purpose

ORF encoding ERK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-human-p16INK4A-P2A-mCherry

Plasmid

#137026

Purpose

donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

Depositor

Chunliang Li

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

Insert

p16INK4A-knockin-homology arm-P2A-mCherry

Use

Tags

P2A-mCherry

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-JNKKTRmRuby2

Plasmid

#59148

Purpose

ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Tags

mRuby2

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-EF1a-i53

Plasmid

#216243

Purpose

Express i53 in mammalian cells as a HDR enhancer

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

i53

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV-SpyTag-sfCherry2(11)-TagBFP

Plasmid

#117485

Purpose

Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyTag002-GG-sfCherry2(11)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3V(1-10)

Plasmid

#117483

Purpose

Lentiviral vector for constitutive expression of sfCherry3V(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3V(1-10)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

6 substitutions (K45R, G52D, L83W, T106A, K182R, …

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTagBFP2-WPRE

Plasmid

#193339

Purpose

AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Use

AAV

Tags

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pArk313

Plasmid

#200257

Purpose

Ark313 rep/cap

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

Ark313 Rep/Cap

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-TadCBEd-ivt-template

Plasmid

#193843

Purpose

Template for in vitro transcription of TadCBEd (with SpCas9)

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SpCas9

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-c-Met

Plasmid

#31786

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV CMV GCGR-V5-sTurboID (N)

Plasmid

#153009

Purpose

Expresses split-TuboID N-terminal fragment fused to GCGR

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

GCGR-sTurboID (N) (L73)

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadDE

Plasmid

#193846

Purpose

Express TadDE (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE with SaCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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