Addgene

Showing: 741 - 760 of 7956 results

Results per page:

Sort results by:

pX330_ACTB-i3 sgRNA / hSpCas9

Plasmid

#172827

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_ACTB-i2 sgRNA / hSpCas9

Plasmid

#172826

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-sgRNA-MS2-U6

Plasmid

#171694

Purpose

To construct expression vector for tBE-V5-mA3

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-MS2-U6

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico_U6-PLOD2 sgRNA3

Plasmid

#136458

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico_U6-PLOD2 sgRNA5

Plasmid

#136460

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico_U6-PGC1a1 sgRNA

Plasmid

#165425

Purpose

Expression of gRNA against human PGC-1a variant 1

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against PGC-1a variant 1 (PPARGC1A Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only
pGL3-U6-sgRNA_FANCF-mcherry

Plasmid

#159786

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human FANCF gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting FANCF gene (FANCF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-XYLT2-sgRNA

Plasmid

#154862

Purpose

Lentiviral expression of Cas9 and sgRNA targeting XYLT2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

XYLT2 sgRNA (XYLT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-Myosin7B-sgRNA

Plasmid

#154861

Purpose

Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

MYO7B sgRNA (MYO7B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA BRCA2 S3070F

Plasmid

#139325

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 S3070F using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA BRCA1 S1363L

Plasmid

#139329

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 S1363L using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA BRCA1 E1754K

Plasmid

#139330

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E1754K using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA FANCD2 Q223Stop

Plasmid

#139332

Purpose

Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert FANCD2 Q223Stop using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA BRCA2 R2896C

Plasmid

#139511

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 E2896C using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL436 HEK293site3EQR sgRNA

Plasmid

#126445

Purpose

To cut the human HEK293 site3 locus for integration.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human HEK293 site3 with NGAG PAM

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

Plasmid

#119070

Purpose

Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

Plasmid

#119071

Purpose

Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled2-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-H1-sgRNA-mTZAP

Plasmid

#87187

Purpose

guideRNA targeting exon2 of mouse TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (Zbtb48 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge