Addgene

We narrowed to 8,352 results for: plat

Showing: 741 - 760 of 8352 results

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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 (SpeI)

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-IVT

Plasmid

#215834

Purpose

Template for in vitro transcription of CBE6b (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pwtCas9-bacteria

Plasmid

#44250

Purpose

aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

wild-type Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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pJAT10

Plasmid

#204293

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Tags

Expression

Mutation

Promoter

MHC

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6a-IVT

Plasmid

#215833

Purpose

Template for in vitro transcription of CBE6a (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAB_PalkB_sfgfp_KanR

Plasmid

#166503

Purpose

Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PalkB

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c-IVT

Plasmid

#215835

Purpose

Template for in vitro transcription of CBE6c (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBbS8c_ddCpf1_final

Plasmid

#212040

Purpose

Bacterial expression of the CRISPRi system for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

ddCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP18-tetR_O

Plasmid

#127021

Purpose

Basic Part O- ORF; TetR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part TetR_o

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-ATP

Plasmid

#225248

Purpose

mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSGAb-km

Plasmid

#121999

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV Puro

Plasmid

#68469

Purpose

Retroviral plasmid with multiple cloning site and puromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1_tdTomato

Plasmid

#198041

Purpose

Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Insert

tdTomato

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Tags

Expression

Mutation

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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