Addgene

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pGGD-T2A-E

Plasmid

#218898

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

T2A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-W-ccdB-Z

Plasmid

#218890

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGA-blue

Plasmid

#196336

Purpose

Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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BB3eN_14*

Plasmid

#98549

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AC

Plasmid

#98551

Purpose

empty BB3 for insertion and overexpression of 2 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AD

Plasmid

#98552

Purpose

empty BB3 for insertion and overexpression of 3 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AE

Plasmid

#98553

Purpose

empty BB3 for insertion and overexpression of 4 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AF

Plasmid

#98554

Purpose

empty BB3 for insertion and overexpression of 5 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AG

Plasmid

#98555

Purpose

empty BB3 for insertion and overexpression of 6 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AI

Plasmid

#98557

Purpose

empty BB3 for insertion and overexpression of 8 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_34_chr4_0883tt

Plasmid

#98524

Purpose

BB1 with insert: chr4_0883tt

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

chr4_0883tt

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_34_IDP1tt

Plasmid

#98525

Purpose

BB1 with insert: IDP1tt

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

IDP1tt

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_34_RPS3tt

Plasmid

#98527

Purpose

BB1 with insert: RPS3tt

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS3tt

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_34_RPL2Att

Plasmid

#98528

Purpose

BB1 with insert: RPL2Att

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPL2Att

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AC

Plasmid

#98530

Purpose

empty BB3 for insertion and overexpression of 2 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AD

Plasmid

#98531

Purpose

empty BB3 for insertion and overexpression of 3 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AE

Plasmid

#98532

Purpose

empty BB3 for insertion and overexpression of 4 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AF

Plasmid

#98533

Purpose

empty BB3 for insertion and overexpression of 5 genes in P. pastoris

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pMDH3

Plasmid

#98503

Purpose

BB1 with insert: promoter PMDH3

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

MDH3 promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pADH2

Plasmid

#98504

Purpose

BB1 with insert: promoter PADH2

Depositor

Diethard Mattanovich, Michael Sauer, Brigitte Gasser

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

ADH2 promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge