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Plasmid#104823PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma17g11235
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg
Plasmid#222826PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan
Plasmid#222827PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar
Plasmid#222828PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg5A
Plasmid#222829PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan5A
Plasmid#222830PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar5A
Plasmid#222831PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC37
Plasmid#104811PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from Gmubi promoterDepositorInsertMedtr3g107390
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
Plasmid#110547PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.DepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A, H840APromoterpBADAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9
Plasmid#154925PurposeaTc inducible eCas9DepositorInserteCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060APromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9-D1135E
Plasmid#154926PurposeaTc inducible eCas9 with D1135E mutationDepositorInserteCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060A, D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-OptiCas9-D1135E
Plasmid#154928PurposeaTc inducible OptiCas9 with D1135E mutationDepositorInsertOptiCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationR661A, K1003H, D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9
Plasmid#154929PurposeaTc inducible HypaCas9DepositorInsertHypaCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698APromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9-D1135E
Plasmid#154930PurposeaTc inducible HypaCas9 with D1135E mutationDepositorInsertHypaCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698A/D1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9-D1135E
Plasmid#154931PurposeaTc inducible Cas9 with D1135E mutationDepositorInsertCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationD1135EPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PAJ379
Plasmid#104143PurposeExpresses 3A igRNA and sfGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAJ407
Plasmid#104144PurposeExpresses 3A_v9 igRNA and sfGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAJ426
Plasmid#104145PurposeExpresses HIV3 igRNA and sfGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar9si
Plasmid#222834PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg9si
Plasmid#222832PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only