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Plasmid#135260PurposeGateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-dsRed
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-PKN3_IDG-K
Plasmid#170601PurposeGateway destination clone of PKN3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-PKN3 (PKN3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K
Plasmid#158160PurposeGateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-3xHA-miniTurbo-3XNLS
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-TLK2-V5_IDG-K
Plasmid#135251PurposeGateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTLK2-V5 (TLK2 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K
Plasmid#158161PurposeGateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-miniTurbo-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-RIOK2_IDG-K
Plasmid#170586PurposeGateway destination clone of RIOK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-RIOK2 (RIOK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-TcRb:mCherry-FKBP
Plasmid#72904PurposeER-targeted mCherry-tagged recruiter constructDepositorInsertT cell receptor β chain
UseLentiviralTagsbiotinylation acceptor peptide (AP) and mCherry, …ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
K560_AviN_coBirA
Plasmid#129766PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NEK7-V5-miniTurbo_IDG-K
Plasmid#189882PurposeGateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK7 (NEK7 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NEK5_IDG-K
Plasmid#189876PurposeGateway destination clone of NEK5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK5 (NEK5 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-TurboID-NES-STOP_IDG-K
Plasmid#158162PurposeGateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-TurboID-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-STK33_IDG-K
Plasmid#170611PurposeGateway destination clone of STK33 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-STK33 (STK33 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-VRK3-V5-miniTurbo_IDG-K
Plasmid#189902PurposeGateway destination clone of VRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertVRK3 (VRK3 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NEK3_IDG-K
Plasmid#198006PurposeGateway destination clone of NEK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-NEK3 (NEK3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NEK11_IDG-K
Plasmid#198005PurposeGateway destination clone of NEK11 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-NEK11 (NEK11 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1A1L_IDG-K
Plasmid#170591PurposeGateway destination clone of CSNK1A1L (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1A1L (CSNK1A1L Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-VRK2_IDG-K
Plasmid#158196PurposeGateway destination clone of VRK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-VRK2 (VRK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-PKMYT1-V5_IDG-K
Plasmid#135248PurposeGateway destination clone of PKMYT1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPKMYT1-V5 (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only