Addgene

We narrowed to 3,798 results for: SPL

Showing: 741 - 760 of 3798 results

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pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS77

Plasmid

#215681

Purpose

Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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NODALvar-MYC-DYK-C302S

Plasmid

#115254

Purpose

For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

MYC-DYK

Expression

Mammalian

Mutation

C302S

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-MYC-DYK-C302A

Plasmid

#115255

Purpose

For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

MYC-DYK

Expression

Mammalian

Mutation

C302A

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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plIVMD163

Plasmid

#160935

Purpose

In vivo mRNA display construct for mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop

Use

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R373

Plasmid

#138243

Purpose

Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GKA).SaP-dpol*.(CEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R362

Plasmid

#138232

Purpose

Expresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(ASC).CbP-RNR*.(CRL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R376

Plasmid

#138246

Purpose

Expresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGG).Pfu-RIR1-1*.(TGL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R363

Plasmid

#138233

Purpose

Expresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NGK).Ckl-Ter*.(TTL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R366

Plasmid

#138236

Purpose

Expresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(AKA).LLP-Pol*.(CNF)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R367

Plasmid

#138237

Purpose

Expresses the split mCherry reporter interrupted by the LP-Hel intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(ATG).LP-Hel*.(CGK)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R368

Plasmid

#138238

Purpose

Expresses the split mCherry reporter interrupted by the MP-B-DnaB artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(QDQ).MP-B-DnaB*.(TKN)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R369

Plasmid

#138239

Purpose

Expresses the split mCherry reporter interrupted by the MP-K-gp53 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NHD).MP-K-gp53*.(SRA)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFP.R370

Plasmid

#138240

Purpose

Expresses the split mCherry reporter interrupted by the MP-C-gp206 artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(ELK).MP-C-gp206*.(TQN)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R372

Plasmid

#138242

Purpose

Expresses the split mCherry reporter interrupted by the PP-Phi artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(AGK).PP-Phi*.(SLT)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFP.R374

Plasmid

#138244

Purpose

Expresses the split mCherry reporter interrupted by the SaP-Hel artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGK).SaP-Hel*.(TSS)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFP.R355

Plasmid

#138225

Purpose

Expresses the split mCherry reporter interrupted by the Cro-RIR1 artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(VNQ).Cro-RIR1*.(SGR)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge