Addgene

We narrowed to 11,898 results for: NUC

Showing: 741 - 760 of 11898 results

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDY-HA1-HA2-neo

Plasmid

#109123

Purpose

FR cassette provider to introduce FR cassette to a BAC by recombineering

Depositor

David Gilbert

Article

Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

Inserts

HA1
HA2

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbmiR164e/h

Plasmid

#231156

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbmiR164e/h

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbmiR164e/h

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_SlMIR164b

Plasmid

#231152

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting SlMIR164b

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting SlMIR164b

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-1pro

Plasmid

#231151

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbATML1-1pro

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbSTM-5'UTR

Plasmid

#231150

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbSTM-5?UTR

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbPDS

Plasmid

#231146

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbPDS

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-2_NbPDS

Plasmid

#231145

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbPDS

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX 318-320A

Plasmid

#192060

Purpose

Expression vector for SOX (ORF37) 318-320A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Use

Tags

Expression

Insect

Mutation

318-320A

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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SOX D221A/E244A/K246A pACEmam1

Plasmid

#192050

Purpose

Expresses a 3X catalytic mutant of SOX (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (3X catalytic mutant)

Use

Unspecified

Tags

Expression

Mutation

3X catalytic mutant D221A/E244A/K246A

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K246A

Plasmid

#192051

Purpose

Expresses a catalytic mutant of SOX (K246A)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K246A)

Use

Unspecified

Tags

Expression

Mutation

Catalytic mutant K246A

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K250A

Plasmid

#192052

Purpose

Expresses a DNA binding mutant K250A (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K250A)

Use

Unspecified

Tags

Expression

Mutation

DNA binding mutant K250A

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) 318-320A

Plasmid

#192054

Purpose

Expresses a mutant of SOX 318-320A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (318-320A)

Use

Unspecified

Tags

Expression

Mutation

SOX 318-320A

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC Renilla Luc SRE

Plasmid

#191657

Purpose

Expressed Renilla Luciferase with SOX resistance element

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

Renilla Luciferase SRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-HGM (optimized)

Plasmid

#220988

Purpose

Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

HaloTag-GFP-mito

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[R126A/R130A]

Plasmid

#221197

Purpose

ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

R126A, R130A

Promoter

T7

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSG7-10xHis-ecRNHI*

Plasmid

#219523

Purpose

bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

Depositor

Arthur Palmer

Article

Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

Insert

cysteine-free variant of the E. coli ribonuclease HI gene

Use

Tags

N terminal 10xHis Tag

Expression

Bacterial

Mutation

Cysteine free variant

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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