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Plasmid#210469PurposeDonor plasmid for knock-in GFP-CD19 into the human INS locusDepositorInsertINS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC-attB-burs-mCD8-EGFP-Gal4
Plasmid#39482DepositorInsertburs-mCD8-EGFP-Gal4
UseTagsExpressionMutationPromoterbursalphaAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-hSPC-TFEB(delta30)eGFP-W
Plasmid#126693PurposeLineage specific over expression of a mutated TFEB in lung alveolar epithelial cells, where TFEB is missing the first 30 amino acids of the sequence.DepositorInsertTFEB(delta30) (TFEB Human)
UseLentiviralTagsExpressionMutationDeleted amino acids 1-30, H32D, Deleted Stop Codo…PromoterhSPCAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA
Plasmid#176461PurposeVector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMVDepositorInsertBbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D122A pcDNA3
Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176459PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP) mnuctdTomato
Plasmid#172342PurposemnuctdTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTagsSV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mouse 3' Hp1a AID GFP PuroR
Plasmid#127897PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a GeneDepositorInsertHp1a (Cbx5 Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' Hp1aPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176460PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-aACry::EGFP-zebrafish Plaat1 (WT)
Plasmid#168772PurposeExpress EGFP-zebrafish Plaat1 (WT) in zebrafish lensDepositorInsertHrasls (plaat1 Zebrafish)
UseTagsEGFPExpressionMutationPromoteralphaA-crystallin promoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CjCas9-eGFP-HIF1a
Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAV and CRISPRTagsSV40NLS-HA-T2A-EGFPExpressionMutationPromoterEF-1alphaAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-GFPi
Plasmid#31848DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN L38&45P
Plasmid#22183DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationChanged Luecine 38 to Proline and Leucine 45 to P…PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX304 Flag-mCherry-eEF1A1_IRES-GFP
Plasmid#198383PurposeeEF1A1 fluorescent reporterDepositorInserteEF1A1 (EEF1A1 Human)
UseLentiviralTagsFlag-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-APPL1-R146A/K152A/R154A
Plasmid#59767PurposeExpresses APPL1 Endosomal Localization MutantDepositorInsertAPPL1 (APPL1 Human)
UseTagsEGFPExpressionMammalianMutationR146A/K152A/R154APromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 DomI + DomII pMT2
Plasmid#206095Purposeexpression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tagDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC-attB-burs-mCD8-EGFP-T2A-Gal4
Plasmid#39463DepositorInsertburs-mCD8-EGFP-T2A-Gal4
UseTagsExpressionMutationPromoterbursalphaAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hDNAJC6-T2A-copGFP
Plasmid#170443PurposeLentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoterDepositorInserthuman DNAJC6 (DNAJC6 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)
Plasmid#22131DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralTagsExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only