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2xHP1bCD_W42A-IFP2.0C

Plasmid

#215751

Purpose

Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD_W42A-IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1A:1-461

Plasmid

#211111

Purpose

Mammalian expression of full-length human CORO1A. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Neo-LUC

Plasmid

#214368

Purpose

Expression of the firefly luciferase Protein

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

LUC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_RPA2:1-270

Plasmid

#211013

Purpose

Mammalian expression of full-length human RPA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RPA2:M1-E270 (RPA2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYSD313

Plasmid

#212931

Purpose

Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

Sed1 (SED1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRPA70-IRES-Ven-RPA32-IRES-RPA14

Plasmid

#211430

Purpose

mammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14

Depositor

Helmut Pospiech

Insert

RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)

Use

Tags

mVenus (on RPA32)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

Plasmid

#211966

Purpose

Express gRNA against JARID2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting JARID2 (JARID2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_SS18_SSX1

Plasmid

#205927

Purpose

Express mEGFP-tagged fusion protein, SS18_SSX1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SS18_SSX1 (SS18, SSX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD18

Plasmid

#208270

Purpose

Production of ent-isokaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

ptmT2
osKSL6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD10

Plasmid

#208262

Purpose

Production of labda-7,13(16),14-triene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

grTPS2
sclav_p0491

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD09

Plasmid

#208261

Purpose

Production of biformene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

cldB
cldD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD05

Plasmid

#208259

Purpose

Production of isopimara-8,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
sare_1287

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD01

Plasmid

#208257

Purpose

Production of terpentetriene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cyc1
cyc2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Hygro

Plasmid

#186671

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Hygromycin (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-R2L3-IRESpacDeltaTKpA

Plasmid

#139535

Purpose

Provides IRES-pacDeltaTK-pA cassette on Gateway entry vector

Depositor

Minoru Ko

Insert

PacTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-MMACHC [N230/21R]

Plasmid

#114555

Purpose

Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Otx1

Plasmid

#84315

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Otx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Otx1 (Otx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(457-924)

Plasmid

#42006

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa457-924

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1-456)

Plasmid

#42005

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-456

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP

Plasmid

#13031

Purpose

Mammalian expression vector for cloning your gene fused to EGFP

Depositor

Doug Golenbock

Insert

Enhanced Green Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mCherry-T2A-Puro

Plasmid

#72264

Purpose

Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

Depositor

Kazuhiro Oka

Insert

mCherry-T2A-Puro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCE-mp53DD

Plasmid

#41856

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp53 (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

Plasmid

#159905

Purpose

Mutagenesis of Slc32a1

Depositor

Larry Zweifel

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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