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Plasmid#91087PurposeModule C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-a2-GEMS(JAK-STAT)
Plasmid#209110PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)DepositorInsertA-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l4-G'-IRES-EmGFP
Plasmid#209127PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFPDepositorInsertSUMO-A'-l4-G'-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l4-G-IRES-EmGFP
Plasmid#209126PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFPDepositorInsertSUMO-A'-l4-G-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
POLR2A-N CRISPR pX330
Plasmid#124495PurposeA CRISPR plasmid for targeting the N-terminus coding region of human POLR2ADepositorInsertPOLR2A targeting CRISPR
UseCRISPRTagsExpressionMutationPromoterU6 promoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 mCit mCer
Plasmid#83563PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPRKCA (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2520
Plasmid#91089PurposeModule C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterOsU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-no-pep
Plasmid#47439Purposecontrol FRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and no…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-q-pep
Plasmid#47437PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2516b
Plasmid#91085PurposeModule C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC4
Plasmid#91182PurposeT-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting 58kb region in medicago truncatula
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC2
Plasmid#91180PurposeT-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting NCR genes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC362
Plasmid#91216Purposeprotoplast vector for targeted deletion of 6 genes in tomatoDepositorInsertgRNAs targeting 6 tomato genes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC3
Plasmid#91181PurposeT-DNA vector for targeted deletion of 58kb region in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting 58kb region in medicago truncatula
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-GEMS(JAK-STAT)
Plasmid#209109PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)DepositorInsertA-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-a3-GEMS(JAK-STAT)
Plasmid#209111PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)DepositorInsertA-a3-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-B-a2-GEMS(JAK-STAT)
Plasmid#209112PurposeTransient mammalian expression of the CC functionalized GEMS receptor B-a2-GEMS (JAK-STAT)DepositorInsertB-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-G-a2-GEMS(JAK-STAT)
Plasmid#209113PurposeTransient mammalian expression of the CC functionalized GEMS receptor G-a2-GEMS (JAK-STAT)DepositorInsertG-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l2-A'-IRES-EmGFP
Plasmid#209125PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFPDepositorInsertSUMO-A'-l2-A'-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only