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Plasmid#157992PurposeLentiviral vector of constitutive expression of mNeonGreen3A(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationW148L, M150V, K153M, N194K, F204LPromoterSFFVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XMS2
Plasmid#75389PurposesgRNA1-2XMS2DepositorInsertsgRNA1-2XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCnCas12f1HS_sgRNA_MS13_empty
Plasmid#204999PurposeCnCas12f1 with MS13 sgRNA site for Human cell genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL SFFV d20GFP.T2A.mTagBFP Donor
Plasmid#31485DepositorInsertSFFV d20BFP T2A BFP
UseLentiviralTagsExpressionMutationGFP has 20 amino acids deleted from the C terminu…PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-NanoLuc
Plasmid#189975PurposePart 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAsDepositorInsertNanoLuc
UseCloningTags3xFLAGExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9-ScaligD
Plasmid#125688PurposeGene knockout/in for actinomycetesDepositorInsertstreptomyces codon optimized spCas9, sgRNA casette, and ScaligD
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_psychLight1
Plasmid#198739PurposeExpresses psychLight1 in mammalian cellsDepositorInsertpsychLight1
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM496 (deletions) Zeo
Plasmid#109301PurposeORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.DepositorInsertBxb1 attB site
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH3.29_Cre
Plasmid#205560PurposeExpress Cre (without export tag)DepositorInsertCre
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHen2 Avi-tag-linker-p3
Plasmid#119819PurposeFor cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophageDepositorInsertlinker with Avi-tag and p3
UseTagsAvi-tagExpressionBacterialMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-iCre
Plasmid#51904PurposeCre recombinaseDepositorInsertimproved Cre recombinase
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta BR delta CT
Plasmid#13257DepositorInsertRepressor Activator Protein 1 delta Br delta CT (TERF2IP Human)
UseRetroviralTagsFLAGExpressionMutationcontains hRap1 aa2-40 + 96-270 +331-399 and the p…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKD236-4b
Plasmid#90956PurposeExpresses ThsS constitutivelyDepositorInsertThsS
UseTagsExpressionBacterialMutationPromoterBba_J23104Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH2.1_mCherry
Plasmid#205520PurposeExpress mCherry (without export tag)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-eNpHR3.0-TS
Plasmid#158703PurposeReporter constructDepositorInsertmCherry-p2A-eNpHR3.0-TS
UseAAVTagsmCherryExpressionMammalianMutationPromoterTREAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXN22-Dest
Plasmid#105085PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubG-3xHAExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …TagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_Ef1a-->flagLaM4_Notch_tTA
Plasmid#162237PurposeLentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cellsDepositorInsertLaM4 mCherry nanobody Notch tTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
reporter-gT1
Plasmid#47320PurposeTF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)DepositorInsertminCMV-dTomato_pA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only