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  11. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  15. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

    Plasmid
    #70231
    Purpose
    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Steven Henikoff
    Article
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70231-plasmid-map-sequence-id-129162

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Showing: 7561 - 7580 of 52568 results