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Plasmid#203741PurposeIntegration of OsTIR1(F74A)-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationCodon 74 changed from Phe to Ala (F74A)PromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
UseTagsstrep tagExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2155
Plasmid#141948PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBCL11A (BCL11A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2WT
Plasmid#101221PurposeBlue-light dependent optogenetic protein Cry2DepositorInsertCry2PHR
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sg1-sg2
Plasmid#192479PurposeAll-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1DepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-R
Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
ilux pGEX(-)
Plasmid#107879PurposeBacterial expression of ilux for bioluminescence imagingDepositorInsertilux
UseTagsExpressionBacterialMutationPromotertacAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP
Plasmid#219639PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only