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pTJK509

Plasmid

#121464

Purpose

2µm HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Tags

Expression

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK508

Plasmid

#121463

Purpose

2µm HIS3 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Tags

Expression

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK516

Plasmid

#121473

Purpose

CEN LEU2 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Tags

Expression

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK506

Plasmid

#121461

Purpose

CEN HIS3 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Tags

Expression

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK505

Plasmid

#121460

Purpose

CEN HIS3 CNB1mutEF3

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Tags

Expression

Mutation

Changed aspartic acid 103 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK504

Plasmid

#121459

Purpose

CEN HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Tags

Expression

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK503

Plasmid

#121458

Purpose

CEN HIS3 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Tags

Expression

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

PRC1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDblet

Plasmid

#8848

Depositor

Joel Huberman

Article

Brun et al Gene. 1995 Oct 16. 164(1):173-7.

Insert

ARS doublet

Use

Yeast cloning vector

Tags

Expression

Yeast

Mutation

This vector contains, between AatII sites, a dire…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)

Plasmid

#106471

Purpose

Expresses Prc1 with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

Promoter

PRC1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMS22L-siR3

Plasmid

#221013

Purpose

mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (MMS22L Human)

Use

Tags

Flag and myc

Expression

Mammalian

Mutation

silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

Promoter

CMV

Available since

July 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin

Plasmid

#223962

Purpose

Dual fluorescent reporter for histone H2B and for exogenous Rb

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)

Use

Tags

YFP and iRFP670

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Use

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pT2iApowriter2(#336)

Plasmid

#184104

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2iU6Apowriter2N(#355)

Plasmid

#184106

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis + in vitro trans…

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi+SP6

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22Apowriter2(#340)

Plasmid

#184105

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only