Addgene

We narrowed to 27,536 results for: eng

Showing: 7501 - 7520 of 27536 results

Results per page:

Sort results by:

pSARSF53-756

Plasmid

#61756

Purpose

NpuDnaEN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaEN11 intein

Use

Tags

H6-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNJB99

Plasmid

#51521

Purpose

Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Zif268:FokI

Use

Gateway entry vector

Tags

NLS

Expression

Mutation

Promoter

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-PesaR-Dlux

Plasmid

#47653

Purpose

PesaR-D promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-D

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Added an additional esa box upstream of -35 site …

Promoter

PesaR-D

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-AlleyCat

Plasmid

#41715

Depositor

William DeGrado, Ivan Korendovych

Article

Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Use

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

F92E

Promoter

T7

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
phRL-TKlk-lin-41_LCS-sm

Plasmid

#26487

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-sm

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
phRL-TKlk-lin-41_LCS-2U

Plasmid

#26543

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-2U

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
phRL-TKlk-lin-41_LCS-2G

Plasmid

#26544

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-2G

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFredB-PTTG1

Plasmid

#16585

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

PTTG1 (PTTG1 Human)

Use

Tags

Expression

Mammalian

Mutation

3' targeting element

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Use

Tags

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pET His10-Draper INT

Plasmid

#126670

Purpose

Expression of His10-Draper intracellular domain for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
C245

Plasmid

#174351

Purpose

Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

rpl3.L (rpl3.L Frog)

Use

In vitro transcription template

Tags

HA

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
C119

Plasmid

#174354

Purpose

CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

gRNA sequence targeting human PABPC1 near stop codon region

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_EGFPligand

Plasmid

#79129

Purpose

Lentiviral vector for surface expressed EGFP (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

EGFP surface ligand

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pnsgRNA

Plasmid

#172717

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

J23119-gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human 7SK

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge