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Plasmid#129383PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFPDepositorInsertmCherry-ApoB-Tag-BFP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-CPZ-LB7 vpr
Plasmid#115834PurposeEncodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance proteinDepositorInsertSIVCPZ-LB7 Vpr (DQ373064)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsubDD-YPet-NES
Plasmid#84633PurposeEncodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsubDD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Rab1a N124I
Plasmid#46778Purposeexpression of N124I Rab1a in mammalian cellsDepositorInsertRab1a (Rab1a Mouse)
UseTagsFLAGExpressionMammalianMutationN124I cannot stably bind guanine nucleotidePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
UseTags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e-dimer
Plasmid#138503PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588D
Plasmid#48194PurposeExpresses L588D mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588A
Plasmid#48193PurposeExpresses L588A mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_R665A
Plasmid#48195PurposeExpresses R665A mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap-SH2
Plasmid#46500DepositorInsertSlap (SLA Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 61-221PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-2/3
Plasmid#91420PurposeProtein expression and purification of human SH3 domain construct NCK1-2/3DepositorInsertNCK1-2/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginC
Plasmid#29505DepositorInsertElongin C (ELOC Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKT3Ts-goldyTALEN
Plasmid#80330PurposeUpdated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequenceDepositorTypeEmpty backboneUseTALENTagsExpressionBacterialMutationPromoterT3Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SUV39h1 ts1
Plasmid#115880PurposeSUV39h1 knockdownDepositorInsertSUV39h1 shRNA (SUV39H1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-myc
Plasmid#20212DepositorInsert2xFKBP (FKBP1A Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only