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Plasmid#91478PurposeProtein expression and purification of human SH3 domain construct UBASH3A-1/1DepositorInsertUBASH3A-1/1 (UBASH3A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-PPHLN1 ts2
Plasmid#115878PurposePPHLN1 knockdownDepositorInsertPPHLN1 shRNA (PPHLN1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PumU
Plasmid#87322PurposePumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind UPromoterUBCAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PumG
Plasmid#87320PurposePumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind GPromoterUBCAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PumA
Plasmid#87319PurposePumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind APromoterUBCAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup93-mut
Plasmid#87335PurposeTo express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP93 (NUP93 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpLexA::mini35s (GB0733)
Plasmid#68180PurposeProvides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid partDepositorInsert4xOpLexA:mini35S
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP-HF
Plasmid#157735Purposemammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP-HF
UseTagsHis8-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e-dimer
Plasmid#138505PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-dLbCas12a
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mCherry-ApoB-TagBFP
Plasmid#129383PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFPDepositorInsertmCherry-ApoB-Tag-BFP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-CPZ-LB7 vpr
Plasmid#115834PurposeEncodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance proteinDepositorInsertSIVCPZ-LB7 Vpr (DQ373064)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsubDD-YPet-NES
Plasmid#84633PurposeEncodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsubDD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Rab1a N124I
Plasmid#46778Purposeexpression of N124I Rab1a in mammalian cellsDepositorInsertRab1a (Rab1a Mouse)
UseTagsFLAGExpressionMammalianMutationN124I cannot stably bind guanine nucleotidePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
UseTags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e-dimer
Plasmid#138503PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap-SH2
Plasmid#46500DepositorInsertSlap (SLA Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 61-221PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588D
Plasmid#48194PurposeExpresses L588D mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only