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pSW179 - pML1

Plasmid

#115977

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Use

Tags

His and myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-ABE

Plasmid

#107723

Purpose

For expression of ABE7.10 in mammalian cells

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Insert

ABE7.10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Ares-Aphrodite

Plasmid

#13571

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Use

Tags

Aphrodite and Ares

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk2 DN HA

Plasmid

#27651

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

D146N (Dominant Negative)

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pmCellFree _KA2_ZIKV NS5

Plasmid

#145368

Purpose

Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

Plasmid

#69224

Purpose

Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS and Zif268

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-Y100F

Plasmid

#110826

Purpose

Bacterial expression of E.coli DHFR Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

Y100 mutated to F100

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Paxillin-22

Plasmid

#57153

Purpose

Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Paxillin

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSV HA eIF2beta WT

Plasmid

#45898

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Eif2s2 (Eif2s2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-L3-SpCas9-HF1-2A-EGFP-L2

Plasmid

#141048

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF1-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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Human SOX17 sgRNA

Plasmid

#59725

Purpose

Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hSox17 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
gRNA_tdtomato_reporter_1_ST1_2x_binding

Plasmid

#79368

Purpose

Assays activity of transcriptional activators fused to ST1 Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-CD4-Sema4D

Plasmid

#51603

Purpose

expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4-Sema4D

Use

Tags

myc

Expression

Mammalian

Mutation

Sema4D amino acids 1-754 have been replaced with …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pGL-RbUTR

Plasmid

#20883

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rb 3'UTR (Rb1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-microRNA 199-3-Dendra2

Plasmid

#97119

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 199 3 Dendra2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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Human BLIMP1 sgRNA

Plasmid

#59724

Purpose

Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hBLIMP1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only