Addgene

Showing: 7481 - 7500 of 148587 results

Results per page:

Sort results by:

pDONR223_CD3E_WT

Plasmid

#81865

Purpose

Gateway Donor vector containing CD3E , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CD3E (CD3E Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
12xMoonTag-12xSunTag-kif18b-24xPP7

Plasmid

#128606

Purpose

Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

12xMoonTag-12xSunTag
kinesin family member 18b (KIF18B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAP4K2

Plasmid

#23644

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K2 (MAP4K2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AktAR2-NES

Plasmid

#175011

Purpose

Cytosol-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-U6-crRNA-empty

Plasmid

#134921

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

crRNA for CRISPR-Cas3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-BsaI

Plasmid

#54340

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only
pBAD-mTagBFP2

Plasmid

#34632

Depositor

Vladislav Verkhusha

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

mTagBFP2

Use

Tags

Expression

Bacterial

Mutation

I174A compared to mTagBFP (enhanced photostabilit…

Promoter

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1777 (pEffector)

Plasmid

#160731

Purpose

Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins and crRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11350

Plasmid

#183207

Purpose

PB-TRE3G-BFP-Triplex-crGFP*ABE3-SV40pA

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-H2A-TORCAR

Plasmid

#175016

Purpose

TORCAR targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-TORCAR

Use

Tags

Histone H2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-hArfaptin1

Plasmid

#206404

Purpose

Mammalian expression of hArfaptin1

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin1 (ARFIP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAPK13

Plasmid

#23739

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK13 (MAPK13 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pETDuet-1 Hepatitis C Virus NS3/NS4A

Plasmid

#61696

Purpose

inducible dual expression of NS3 protease along with its cofactor NS4A

Depositor

Michael Johnson

Article

Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

Inserts

NS4A
NS3

Use

Tags

his

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM200

Plasmid

#13079

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

Insert

Ptac-gam-bet-exo operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7xTS-sfGFP

Plasmid

#117390

Purpose

Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge