Addgene

We narrowed to 18,164 results for: omp

Showing: 7461 - 7480 of 18164 results

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pcDNA5/FRT/TO DNAJA3

Plasmid

#19466

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJA3 (DNAJA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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A3H HapII-Cas9n-UGI

Plasmid

#119141

Purpose

Base editor made from APOBEC3H Haplotype II

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II-Cas9 nickase-UGI

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP (F64L, S65T, V163A)-TRP1

Plasmid

#53184

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Expression

Mutation

Promoter

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pBacPAK8-ElonginB

Plasmid

#29504

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin B (ELOB Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pEGB Pnos:Luciferase:Tnos (GB0112)

Plasmid

#68217

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VER9063 vpr

Plasmid

#115825

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver 9063)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-N

Plasmid

#170153

Purpose

AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-RESCUE-DDN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPTAN1-1/1

Plasmid

#91292

Purpose

Protein expression and purification of human SH3 domain construct SPTAN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SPTAN1-1/1 (SPTAN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-TAZ CA

Plasmid

#79504

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WWTR1 (WWTR1 Human)

Use

Multisite gateway entry clone

Tags

3xFLAG

Expression

Mutation

S89A

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-FOP

Plasmid

#136797

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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vsv-CenpB 1-166-Sgo1-GFP

Plasmid

#108495

Purpose

expression of vsv-CenpB-1-166-Sgo1-EGFP

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBASH3A-1/1

Plasmid

#91478

Purpose

Protein expression and purification of human SH3 domain construct UBASH3A-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

UBASH3A-1/1 (UBASH3A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PPHLN1 ts2

Plasmid

#115878

Purpose

PPHLN1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PPHLN1 shRNA (PPHLN1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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PumU

Plasmid

#87322

Purpose

PumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pumilio protein mutant (PUM1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

all 8 units mutated to bind U

Promoter

UBC

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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PumG

Plasmid

#87320

Purpose

PumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pumilio protein mutant (PUM1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

all 8 units mutated to bind G

Promoter

UBC

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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PumA

Plasmid

#87319

Purpose

PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pumilio protein mutant (PUM1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

all 8 units mutated to bind A

Promoter

UBC

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP-HF

Plasmid

#157735

Purpose

mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP-HF

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LbABE8e-dimer

Plasmid

#138505

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dLbCas12a

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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