Addgene

We narrowed to 13,073 results for: ung

Showing: 7441 - 7460 of 13073 results

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pC0040-LwaCas13a crRNA backbone

Plasmid

#103851

Purpose

Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-LwaCas13aDR-BbsI-BbsI-polyT

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29R

Plasmid

#17602

Purpose

Mammalian expression of HA tagged ubiquitin K29R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K29R; V5 deleted

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
HBV 1.3-mer X-null replicon

Plasmid

#65461

Purpose

containing 1.3 units of the X-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

Insert

1.3 units of the X-null HBV genome

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP-CAAX

Plasmid

#75149

Purpose

Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P1TDPGH023

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh; U6

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Nanog5P reporter

Plasmid

#16337

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Insert

Nanog promoter (Nanog Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Mouse Nanog promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pC020 - LseCas13a from Listeria seeligeri

Plasmid

#91910

Purpose

Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LseCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-OSBP-PH

Plasmid

#49571

Purpose

expresses the plekstrin homology domain of OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

OSBP-PH (AA 91-179) (OSBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Use

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Promoter

Available since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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#1.4 post-G-SynapShot-mScarlet

Plasmid

#214716

Purpose

Expresses green #1.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-G-SynapShot

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713

Plasmid

#167908

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos36)GFP

Plasmid

#62937

Purpose

Expression of 6xHis-(+36)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(pos36)GFP

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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p6644 MSCV-P C-Flag HA 45E7-Kozak

Plasmid

#35135

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV45 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only