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Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJA1
Plasmid#19464DepositorInsertDNAJA1 (DNAJA1 Human)
UseTagsExpressionMammalianMutationD244GPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-Myc-hAtg14
Plasmid#24293DepositorInsertautophagy related genes 14 (ATG14 Human)
UseTags3xMycExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miRE_shBRD9_561-SV40-GFP
Plasmid#75139PurposeRetroviral expression plasmid encoding shBRD9_561 (targeting human BRD)DepositorInsertshBRD9_561
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIC291
Plasmid#51403PurposeRetroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)DepositorInsertSka1 IMAGE 3909951 (SKA1 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB9
Plasmid#19478DepositorInsertDNAJB9 (DNAJB9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-LU
Plasmid#160700PurposeExpresses GFP-BIRC3 with the long 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISH-Emx2
Plasmid#74346PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.DepositorInsertempty spiracles homeobox 2 (Emx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1omega2_SF (GB0466)
Plasmid#68243PurposeTwister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SF (GB0107)
Plasmid#68233PurposeTwister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro
Plasmid#192681PurposeLentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcriptionDepositorInsertMouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_V591A_S602D
Plasmid#48190PurposeExpresses V591A and S602D mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_V591A_L686E
Plasmid#48191PurposeExpresses V591A and L686E mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_S602D_L686E
Plasmid#48192PurposeExpresses S602D and L686E mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Aps-SH2
Plasmid#46404DepositorInsertAps (SH2B2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 404-523PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(N)-SH2
Plasmid#46520DepositorInsertSyk(N) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-114PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-Flag-YAP2
Plasmid#27472DepositorInsertYes-kinase associated protein (YAP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SNX33-1/1
Plasmid#91278PurposeProtein expression and purification of human SH3 domain construct SNX33-1/1DepositorInsertSNX33-1/1 (SNX33 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only