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Plasmid#183084Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Magenta
Plasmid#183140PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_MagentaDepositorInsertRBS, mRFP1E_Magenta
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Yellow
Plasmid#183141PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_YellowDepositorInsertRBS, mRFP1E_Yellow
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Orange
Plasmid#183143PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_OrangeDepositorInsertRBS, mRFP1E_Orange
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Pink
Plasmid#183142PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_PinkDepositorInsertRBS, mRFP1E_Pink
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
sCatch-GFP
Plasmid#186902PurposeCell-free expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10DepositorInsertsfGFP-SpyCatcher003-sfCherry1-10
UseSynthetic BiologyTagssfGFPExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_rHCaMP
Plasmid#187107PurposeExpression of rHCaMP in mammalian cellsDepositorInsertrHCaMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV-Camk2-ChroMEd15-mTFP
Plasmid#184240Purposechannelrhodopsin ChroME fused to mTFPDepositorInsertChroMEd15-mTFP
UseLentiviralTagsmTFP1ExpressionMammalianMutationdeleted ChroME amino acids 317-331PromoterCamk2Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcS-NP-C1C2
Plasmid#178427PurposeNon peptide (NP) as negative control fused to EV membrane binging domain (C1C2)DepositorInsertNP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sticky-173a-16X
Plasmid#165453PurposeDNA fragment production for nucleosome preparationDepositorTypeEmpty backboneUseBacterial vector for dna productionTagsExpressionMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_18bp
Plasmid#174852PurposeExpress forward pegRNA mediating 991-bp deletion and 18-bp insertionDepositorInsertHEK3_pegF_18bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegR_18bp
Plasmid#174853PurposeExpress reverse pegRNA mediating 991-bp deletion and 18-bp insertionDepositorInsertHEK3_pegR_18bp
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_44bp
Plasmid#174854PurposeExpress forward pegRNA mediating 991-bp deletion and 44-bp insertionDepositorInsertHEK3_pegF_44bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegR_44bp
Plasmid#174855PurposeExpress forward pegRNA mediating 991-bp deletion and 44-bp insertionDepositorInsertHEK3_pegR_44bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzpHS2
Plasmid#172971PurposepWSK29 with S. flexneri wzzpHS2 geneDepositorInsertwzzPHS2 gene from Shigella flexneri
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBSF
Plasmid#172967PurposepWSK29 with S. flexneri 2a wzzBSF geneDepositorInsertwzzB gene from Shigella flexneri
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS4110
Plasmid#131743Purposec-di-GMP-II switch cdGII-CGUAADepositorInsertc-di-GMP-II switch cdGII-CGUAA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS4102
Plasmid#131742PurposeXanthine switch Xan-ACGAGDepositorInsertXanthine switch Xan-ACGAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sticky-171a-16X
Plasmid#165452PurposeDNA fragment production for nucleosome preparationDepositorTypeEmpty backboneUseBacterial vector for dna productionTagsExpressionMutationPromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sticky-nonpalindromic-169an-4X
Plasmid#165413PurposeDNA fragment production for nucleosome preparationDepositorTypeEmpty backboneUseBacterial vector for dna productionTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only