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pLNHA-C1-HsNot3-del607-634_T

Plasmid

#147626

Purpose

Mammalian Expression of HsNot3-del607-634

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del607-634 (CNOT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3-del632-654_T

Plasmid

#147627

Purpose

Mammalian Expression of HsNot3-del632-654

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del632-654 (CNOT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvG-HsNot3_656-753_T

Plasmid

#147619

Purpose

Bacterial Expression of HsNot3_656-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_656-753 (CNOT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsNot3_589-753_T

Plasmid

#147620

Purpose

Bacterial Expression of HsNot3_589-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_589-753 (CNOT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsNot3_656-753_T

Plasmid

#147621

Purpose

Bacterial Expression of HsNot3_656-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_656-753 (CNOT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsNot3_589-753_M

Plasmid

#146947

Purpose

Bacterial Expression of HsNot3_589-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_589-753 (CNOT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_005

Plasmid

#180555

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Tags

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ E. coli, Synthetic)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Expression

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-HistoneH2B-L2

Plasmid

#186357

Purpose

Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Histone H2B (H2BC11 Human)

Use

Expression of a tagged nuclear marker

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_047

Plasmid

#180551

Purpose

Entry vector containing sfGFP in E1.3a format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP in E1.3a format

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_110

Plasmid

#180554

Purpose

Entry vector containing 7xhis tag in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

7xhis tag in E1.3b format

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_007

Plasmid

#180563

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_044

Plasmid

#180532

Purpose

Entry vector containing J23117 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23117 anderson promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_043

Plasmid

#180531

Purpose

Entry vector containing J23101 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23101 anderson promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_031

Plasmid

#180556

Purpose

Entry vector containing Terminator - BBa_B0010 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator - BBa_B0010 terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_032

Plasmid

#180528

Purpose

Entry vector containing pLac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLac promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_045

Plasmid

#180543

Purpose

Entry vector containing RBS 30,000 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 30,000 a.u. designed strength

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_034

Plasmid

#180526

Purpose

Entry vector containing pLux promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLux promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_252

Plasmid

#180553

Purpose

Entry vector containing mScarlet in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet in E1.3b format

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge