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pTK-EdnrbE2-Citrine
Plasmid#127770PurposeEnhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activityDepositorInsertednrbE2
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Trex2-bpA
Plasmid#86984PurposeExpression of mouse Trex2DepositorInsertmouse Trex2 coding region (Trex2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-mCherry
Plasmid#127772PurposeEnhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activityDepositorInsertednrbE2
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK-RetE1-Citrine
Plasmid#127779PurposeEnhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activityDepositorInsertRetE1
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 G120A
Plasmid#123076PurposeGateway entry clone encoding human MAP1LC3B2 G120ADepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 120 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-2xMARS-PLD
Plasmid#205237PurposeExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMFDepositorInsertsUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald
Plasmid#201188PurposeLentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-Cerulean
Plasmid#127771PurposeEnhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activityDepositorInsertednrbE2
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE1-Citrine
Plasmid#127773PurposeEnhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activityDepositorInsertednrbE1
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cdc2-DN-HA
Plasmid#1889DepositorInsertCdc2 (CDK1 Human)
UseTagsHAExpressionMammalianMutationD146N, dominant negativePromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-2xMARS-nGFP-puro
Plasmid#205238PurposeLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobodyDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseLentiviralTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.19_mCherry-MS2x2
Plasmid#205534PurposeExpress mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαq*-BERKY3
Plasmid#158217PurposeBRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13*-BERKY3
Plasmid#158218PurposeBRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gβγ-BERKY3
Plasmid#158426PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Rho*-BERKY3
Plasmid#158220PurposeBRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only
